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Analyse quantitative de l'autophagie en utilisant avancée Fluorescence Microscopy 3D

Article DOI: 10.3791/50047-v • 09:59 min • May 3rd, 2013
May 3rd, 2013 •
1,2, 2, 2,3, 4,5, 5,6, 1,2,5
1Department of Biochemistry and Molecular Medicine, University of California, Davis, 2NSF Center for Biophotonics Science & Technology, University of California, Davis, 3University of Tromsø, 4Department of Surgery (Division of Surgical Oncology), University of California, Davis, 5UC Davis Comprehensive Cancer Center, University of California, Davis, 6Department of Biological Chemistry, University of California, Davis

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L'autophagie est un processus omniprésents qui permet aux cellules de se dégradent et recyclent des protéines et des organites. Nous appliquons la microscopie par fluorescence de pointe pour visualiser et quantifier les petits, mais les changements essentiels, physiques associés à l'induction de l'autophagie, y compris la formation et la distribution de autophagosomes et des lysosomes, et leur fusion dans autolysosomes.

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Biologie cellulaire numéro 75 biochimie biologie moléculaire médecine biologie du cancer biophysique biologie chimique protéines microscopie fluorescence l'autophagie l'arginine déiminase cancer de la prostate la microscopie à déconvolution microscopie imagerie des cellules vivantes structuré éclairage super-résolution tumeurs autophagosomes les lysosomes les cellules la culture cellulaire la microscopie l'imagerie la visualisation
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