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定量高通量单细胞的细胞毒性T细胞的测定
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Immunology and Infection
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JoVE Journal Immunology and Infection
Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells
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定量高通量单细胞的细胞毒性T细胞的测定

DOI:
10.3791/50058-v

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09:28 min

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February 02, 2013

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, , , ,
1Department of Chemical and Biomolecular Engineering,University of Houston , 2Division of Pediatrics, Research Unit 907,University of Texas MD Anderson Cancer Center

Chapters

  • 00:05Title
  • 01:32Fabrication of Arrays of Nanowells in PDMS
  • 02:14Target Cell (T) Labeling
  • 02:53Effector (E) Cell Labeling
  • 03:26Separation of Live and Dead Cells by Ficoll Density Gradient
  • 05:23Cell Loading onto Nanowell Array
  • 06:51Imaging
  • 07:58Results: A High-throughput Cytolytic Assay
  • 09:03Conclusion

Summary

Automatic Translation

Automatic Translation

我们描述了一种单细胞的高通量的检测，以测量与肿瘤靶细胞的培养时的细胞毒性T细胞。此方法采用致密的，弹性体子纳升井（〜100,000井/数组）的数组，在空间上局限在T细胞和靶细胞，在规定的比例，和被耦合到的荧光显微镜来监测效靶共轭和随后的细胞凋亡。

Tags

Single-cell Cytotoxicity AssayT CellsCancer ImmunotherapyAdoptive Cell TherapyCAR T CellsCytotoxicityHigh-throughputNanowell ArrayCell-based AssayTime-lapse Microscopy

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