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Chapters
- 00:05Title
- 02:38Sperm Sample Preparation by the Swim-up Method
- 04:52Fluorescent Dye Loading for Ca2+ Measurements
- 05:41Conventional Fluorometry
- 06:59Stopped Flow Fluorometry
- 08:30Flow Cytometry
- 09:40Single Cell Imaging
- 12:11Results: Progesterone Induces Intracellular Ca2+ Changes in Human Sperm Detected by Fluorometric Techniques
- 17:59Conclusion
Intracellular Ca<sup> 2 +</sup> Dynamik er meget vigtige i sperm fysiologi og Ca<sup> 2 +</sup>-Sensitive fluorescerende farvestoffer udgør et alsidigt værktøj til at studere dem. Population eksperimenter (fluorometri og stoppet flow fluorometri) og encellede eksperimenter (flowcytometri og enkelt celle billeddannelse) bruges til at spore spatio-temporale [Ca<sup> 2 +</sup>] Ændringer i humane sædceller.
