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可视化和分析的mRNA分子，使用荧光<em>在原位</em>杂交<em>酿酒酵母（Saccharomyces cerevisiae）</em
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Visualization and Analysis of mRNA Molecules Using Fluorescence
In Situ
Hybridization in
Saccharomyces cerevisiae
Please note that all translations are automatically generated.
Click here for the English version.
可视化和分析的mRNA分子，使用荧光<em>在原位</em>杂交<em>酿酒酵母（Saccharomyces cerevisiae）</em
DOI:
10.3791/50382-v
•
07:00 min
•
June 14, 2013
•
R. Scott McIsaac
2
,
Sanford J. Silverman
,
Lance Parsons
,
Ping Xu
,
Ryan Briehof
,
Megan N. McClean
,
David Botstein
3
1
The Lewis-Sigler Institute for Integrative Genomics
,
Princeton University
,
2
Graduate Program in Quantitative and Computational Biology
,
Princeton University
,
3
Department of Molecular Biology
,
Princeton University
Chapters
00:05
Title
01:11
Coverslip Preparation
01:51
Fixation
03:08
Hybridization
04:53
Imaging of Cells with Olympus IX-81 Inverted Microscope
05:23
Results: Quantitative mRNA Analysis by FISH
06:31
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本协议描述的实验过程进行荧光<em在原位</em>杂交（FISH）技术在单细胞单分子分辨率计数的mRNA。
Tags
Keywords: Fluorescence In Situ Hybridization
FISH
MRNA
Saccharomyces Cerevisiae
Single-cell Analysis
Single-molecule
Spot Detection
Gene Expression
Visualization
Quantification
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