Journal
/
Biology
/
Flow cytometri af bimolekylær Fluorescens Complementation: En High Throughput Kvantitativ metode til at studere protein-protein interaktion
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction

Flow cytometri af bimolekylær Fluorescens Complementation: En High Throughput Kvantitativ metode til at studere protein-protein interaktion

DOI:
10.3791/50529-v

•

11:11 min

•

August 15, 2013

•

,
1Department of Pharmacology,University of Illinois at Chicago

Chapters

  • 00:05Title
  • 02:05Cell Transfection
  • 03:46Cell Preparation for Flow Cytometry, Western Blot, and Immunofluorescence
  • 05:12Flow Cytometry and Immunofluorescence Microscopy
  • 06:50Data Analysis
  • 07:49Assessing AKAP-Lbc and PDE4D3 Interaction Using BiFC-Flow Cytometry
  • 10:17Conclusion

Summary

Automatic Translation

Automatic Translation

Flowcytometrisk analyse af bimolekylær Fluorescens komplementation giver en høj produktion kvantitativ metode til at studere protein-protein-interaktion. Denne metode kan anvendes til kortlægning proteinbindingssteder og til screening faktorer, der regulerer protein-protein-interaktion.

Tags

Keywords: Flow CytometryBimolecular Fluorescence ComplementationBiFCProtein-protein InteractionAKAP-LbcPDE4D3Fluorescent ProteinHigh ThroughputQuantification

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/50529?article_type=v

A Neuronal and Astrocyte Co-Culture Assay for High Content Analysis of Neurotoxicity
check_url/50529?article_type=v

A high-throughput method to globally study the organelle morphology in S. cerevisiae
check_url/50529?article_type=v

Bimolecular Fluorescence Complementation (BiFC) Assay for Protein-Protein Interaction in Onion Cells Using the Helios Gene Gun
check_url/50529?article_type=v

Quantification of dsDNA using the Hitachi F-7000 Fluorescence Spectrophotometer and PicoGreen Dye
check_url/50529?article_type=v

Bimolecular Fluorescence Complementation
check_url/50529?article_type=v

Detection of Protein Interactions in Plant using a Gateway Compatible Bimolecular Fluorescence Complementation (BiFC) System
check_url/50529?article_type=v

En væskefase affinitetsindfangning under anvendelse af magnetiske perler til at studere protein-protein interaktion: poliovirus-Nanobody Eksempel
check_url/50529?article_type=v

Kvantitativ analyse af Autophagy hjælp Advanced 3D fluorescensmikroskopi
check_url/50529?article_type=v

En High Throughput MHC II Bindende Assay til kvantitativ analyse af peptidepitoper
check_url/50529?article_type=v

Protein-protein interaktioner visualiseret ved bimolekylær Fluorescens komplementering i tobaksprotoplaster og blade

Read Article