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Transsynaptic는 콜레라 독소와 바이오틴 덱스 트란 아민 두 번 라벨로 이어 (僞) 바이러스에 주변 대상에서 추적
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Transsynaptic Tracing from Peripheral Targets with Pseudorabies Virus Followed by Cholera Toxin and Biotinylated Dextran Amines Double Labeling
DOI:

13:12 min

September 14, 2015

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Chapters

  • 00:05Title
  • 01:29Injection of PRV into Mice
  • 04:04Cranial Injection of Biotinylated Dextran Amines
  • 06:41Detection of BDA and CTb in the Same Sections
  • 09:08PRV-infected Neurons and BDA and CTb Circuit Confirmation
  • 12:31Conclusion

Summary

Automatic Translation

Transsynaptic tracing has become a powerful tool for analyzing central efferents regulating peripheral targets through multi-synaptic circuits. Here we present a protocol that exploits the transsynaptic pseudorabies virus to identify and localize a functional brain circuit, followed by classical tract tracing techniques to validate specific connections in the circuit between identified groups of neurons.

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