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幻灯片制备方法，以保存睾丸细菌细胞的三维染色质结构
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Biology
Slide Preparation Method to Preserve Three-dimensional Chromatin Architecture of Testicular Germ Cells
Please note that all translations are automatically generated.
Click here for the English version.
幻灯片制备方法，以保存睾丸细菌细胞的三维染色质结构
DOI:
10.3791/50819-v
•
07:34 min
•
January 10, 2014
•
Satoshi H. Namekawa
2
1
Division of Reproductive Sciences, Division of Developmental Biology, Perinatal Institute
,
Cincinnati Children’s Hospital Medical Center
,
2
Department of Pediatrics
,
University of Cincinnati College of Medicine
Chapters
00:05
Title
01:54
3D Slide Preparation
04:03
Cot-1 RNA FISH
06:08
Results: 3D Chromatin Architecture
07:09
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这里的材料描述了一种为保存睾丸生殖细胞的三维染色质结构而开发的方法。这被称为三维（3D）滑动方法。该方法提高了对亚核结构检测的灵敏度，适用于
原位
杂交（FISH）中的免疫荧光、DNA和RNA荧光。
Tags
Three-dimensional Chromatin Architecture
Testicular Germ Cells
Slide Preparation Method
Permeabilization
Fixation
Cytospin
Immunofluorescence
DNA FISH
RNA FISH
Cot-1 RNA FISH
Germ Cell Differentiation
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