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Journal
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Bioengineering
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FIBS功能的无创性代谢轮廓
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
FIBS-enabled Noninvasive Metabolic Profiling
Please note that all translations are automatically generated.
Click here for the English version.
FIBS功能的无创性代谢轮廓
DOI:
10.3791/51200-v
•
09:16 min
•
February 03, 2014
•
Alireza Behjousiar
2
,
Antony Constantinou
,
Karen M. Polizzi
,
Cleo Kontoravdi
1
Centre for Process Systems Engineering, Department of Chemical Engineering and Chemical Technology
,
Imperial College London
,
2
Centre for Synthetic Biology and Innovation, Division of Molecular Biosciences
,
Imperial College London
Chapters
00:05
Title
01:18
Cell Line Revival and Maintenance
02:06
Cell Line Transfection with the Biosensor Gene-containing Plasmid
03:47
Batch and Fed-batch Cell growth Curve
04:44
FRET Ratio Measurements
05:36
Metabolite Assays
06:08
Results: Representative Fed-batch Culture and Corresponding FRET Measurements
08:54
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
如何
在原位
代谢轮廓校正共振能量转移集成生物传感器(FIBS)用于描述呈现。该FIBS可用于测量非侵入性代谢物辅助的代谢模式和生物过程条件的高通量筛选的发展的细胞内水平。
Tags
FIBS
Noninvasive Metabolic Profiling
Computational Biology
High-throughput Experimental Systems
In Situ Monitoring
Fluorescent Protein-based In Vivo Biosensors
FRET
FRET Integrated Biological Sensors
CHO Cell Lines
Glucose
Glutamine
Intracellular Metabolite Concentration
Fed-batch Cell Culture
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