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En hurtig og konkret Microplate Assay til bestemmelse af intra-og ekstracellulære Ascorbat i dyrkede celler
JoVE Journal
Biology
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JoVE Journal Biology
A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
DOI:

11:56 min

April 11, 2014

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Chapters

  • 00:05Title
  • 02:01Determine Intracellular Ascorbate in Cultured Cells
  • 04:16Determine the Amount of Ascorbate Released and Extracellular Ascorbate
  • 06:25Results: Determination of Intracellular and Extracellular Ascorbate in Cultured Cells
  • 11:36Conclusion

Summary

Automatic Translation

Ascorbat spiller mange vigtige roller i cellulære stofskifte, hvoraf mange har kun kommet frem i de seneste år. Her beskriver vi en medium-throughput, specifik og billig mikroplade assay til bestemmelse af både intra-og ekstracellulære ascorbat i cellekultur.

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