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Kvantificering af Breast Cancer Cell invasiv Ved hjælp af en tre-dimensionel (3D) Model
JoVE Journal
Medicine
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JoVE Journal Medicine
Quantification of Breast Cancer Cell Invasiveness Using a Three-dimensional (3D) Model
DOI:

08:08 min

June 11, 2014

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Chapters

  • 00:05Title
  • 01:063D Culture of Breast Cancer Cells in Basement Membrane Matrix
  • 03:58Examination of Morphogenic Features of 3D Cultures with Immunofluorescence
  • 06:17Results: Representative 3D MDA-MB-231 Cell Basement Membrane Matrix Invasion Images
  • 07:33Conclusion

Summary

Automatic Translation

Denne artikel indeholder detaljerede metoder til anvendelse af tre-dimensionelle (3D) assays at kvantificere brystkræft celle invasion. Konkret har vi diskutere de procedurer, der kræves for at oprette sådanne assays, kvantificering og dataanalyse, samt metoder til at undersøge tabet af membranintegritet der opstår, når celler invadere.

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