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1School of Medical and Molecular Biosciences, Faculty of Science,University of Technology, Sydney, 2Centre for Health Technologies, Faculty of Engineering and Information Technology,University of Technology, Sydney, 3The Sydney Head and Neck Cancer Institute, Sydney Cancer Centre,Royal Prince Alfred Hospital
Chapters
- 00:05Title
- 01:50Small RNA Isolation from Serum
- 03:56RNA Resuspension into solution
- 04:38Results: Marked Improvements on the Traditional Chomczynski Method
- 06:08Conclusion
Este protocolo describe un método para la extracción de ARN pequeños a partir de suero humano. Hemos utilizado este método para aislar los microARNs de suero de cáncer para su uso en arrays de ADN y PCR cuantitativa singleplex. El protocolo utiliza fenol y tiocianato de guanidina reactivos con modificaciones para producir ARN de alta calidad.
