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小非编码RNA从人血清中分离
JoVE Journal
Biology
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JoVE Journal Biology
Isolation of Small Noncoding RNAs from Human Serum
DOI:

06:44 min

June 19, 2014

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Chapters

  • 00:05Title
  • 01:50Small RNA Isolation from Serum
  • 03:56RNA Resuspension into solution
  • 04:38Results: Marked Improvements on the Traditional Chomczynski Method
  • 06:08Conclusion

Summary

Automatic Translation

本协议描述为从人血清中提取的小分子RNA的方法。我们已经用这种方法来隔离患者血清小分子RNA在DNA阵列使用,也可单重定量PCR。该协议利用酚和异硫氰酸胍试剂进行了修改,以产生高质量的RNA。

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