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小非编码RNA从人血清中分离
JoVE Journal
Biology
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JoVE Journal
Biology
Isolation of Small Noncoding RNAs from Human Serum
Please note that all translations are automatically generated.
Click here for the English version.
小非编码RNA从人血清中分离
DOI:
10.3791/51443-v
•
06:44 min
•
June 19, 2014
•
Samantha Khoury
,
Pamela Ajuyah
,
Nham Tran
3
1
School of Medical and Molecular Biosciences, Faculty of Science
,
University of Technology, Sydney
,
2
Centre for Health Technologies, Faculty of Engineering and Information Technology
,
University of Technology, Sydney
,
3
The Sydney Head and Neck Cancer Institute, Sydney Cancer Centre
,
Royal Prince Alfred Hospital
Chapters
00:05
Title
01:50
Small RNA Isolation from Serum
03:56
RNA Resuspension into solution
04:38
Results: Marked Improvements on the Traditional Chomczynski Method
06:08
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本协议描述为从人血清中提取的小分子RNA的方法。我们已经用这种方法来隔离患者血清小分子RNA在DNA阵列使用,也可单重定量PCR。该协议利用酚和异硫氰酸胍试剂进行了修改,以产生高质量的RNA。
Tags
Small Noncoding RNAs
MicroRNAs
Serum
RNA Isolation
Phase Lock Gel
Biomarkers
Gene Regulation
DNA Arrays
QPCR
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