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Journal
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Immunology and Infection
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可扩展的高通量选择从噬菌体展示合成抗体库
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
Please note that all translations are automatically generated.
Click here for the English version.
可扩展的高通量选择从噬菌体展示合成抗体库
DOI:
10.3791/51492-v
•
12:55 min
•
January 17, 2015
•
Shane Miersch
2
,
Zhijian Li
2
,
Rachel Hanna
2
,
Megan E. McLaughlin
2
,
Michael Hornsby
3
,
Tet Matsuguchi
3
,
Marcin Paduch
4
,
Annika Sääf
4
,
Jim Wells
3
,
Shohei Koide
4
,
Anthony Kossiakoff
4
,
Sachdev S. Sidhu
2
1
The Recombinant Antibody Network
,
2
The Banting and Best Department of Medical Research
,
University of Toronto
,
3
Antibiome Center
,
University of California, San Francisco at Mission Bay
,
4
Department of Biochemistry and Molecular Biology
,
The University of Chicago
Chapters
00:05
Title
02:17
Preparing Cells for Phage Selection
03:28
Library Pre-clearance and Antigen-phage Incubation
05:45
Phage Elution and Amplification
08:03
Characterization by Pooled ELISA
10:22
Anticipated Results and Troubleshooting
12:18
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
一种方法是描述视觉伴奏用于同时进行可扩展,高吞吐量选择从噬菌体展示的组合合成的抗体文库针对数百抗原。使用这种平行的方式,我们已经分离,表现出对于不同的抗原有功能在标准免疫高亲和力和特异性的抗体片段。
Tags
Phage Display
Synthetic Antibody Libraries
High-throughput Selection
Fab Fragments
Antigen Characterization
Antibody Discovery
Immunoassays
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