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Chapters
- 00:05Title
- 02:57Induction of Differentiation in Female Embryonic Stem Cells to Induce X Chromosome Inactivation
- 04:55Fixation of Differentiated ES Cells for Subsequent DNA-RNA FISH Analysis
- 05:33Labeling of DNA Probes for FISH Experiments
- 08:02Permeabilization, Pre-treatment and Hybridization of Fixed Cells for Combined DNA-RNA FISH
- 11:04Post-hybridization Washes and Antibody Mediated Detection of Probe
- 13:21Results: X Chromosome Inactivation in Differentiating Female ES Cells is Visualized by Combined DNA-RNA FISH
- 14:57Conclusion
Fluorescerende in situ-hybridisering (FISH) muliggør påvisning af nukleinsyrer i deres native miljø i cellerne. Vi her beskrive en protokol for den kombinerede, samtidig påvisning af RNA og DNA ved hjælp af fisk, som kan bruges til at studere X-kromosom inaktivering i muse embryonale stamceller.
