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10:35 min
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September 06, 2014
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Summary
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Memeli Doku biyopsilerinden mitokondri hızlı izole edilmesi için bir yöntem tarif edilmektedir. Sıçan karaciğer, iskelet kası ya da preparat, ticari ayrıştırıcı doku homojenize edildi ve mitokondri naylon gözenekli filtrelerden geçerek, süzme ile izole edilmiştir. Seçenek yöntemler kullanılarak da 100 dakika - Mitokondriyal izolasyon süresi 60 kıyasla <30 dk.
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Cite this Article
Preble, J. M., Pacak, C. A., Kondo, H., MacKay, A. A., Cowan, D. B., McCully, J. D. Rapid Isolation And Purification Of Mitochondria For Transplantation By Tissue Dissociation And Differential Filtration. J. Vis. Exp. (91), e51682, doi:10.3791/51682 (2014).
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