Быстрый Люминесцентная<em> На месте</em> Гибридизация Протокола о Xist РНК в сочетании с Иммунофлуоресценции модификации гистонов в X-хромосоме инактивации
We developed an easily customized strand-specific fluorescent in situ hybridization (FISH) protocol combined with immunofluorescence. This allows for a detailed examination of RNA dynamics with simultaneous insight into the chromatin structure, nuclear organization, and transcriptional regulation at the single cell level.