Veloce fluorescente<em> In Situ</em> Protocollo di ibridazione per Xist RNA combinata con immunofluorescenza di istone modifica in cromosoma X inattivazione
We developed an easily customized strand-specific fluorescent in situ hybridization (FISH) protocol combined with immunofluorescence. This allows for a detailed examination of RNA dynamics with simultaneous insight into the chromatin structure, nuclear organization, and transcriptional regulation at the single cell level.