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Bioengineering
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同心凝胶系统来研究基质微环境的三维细胞迁移的生物物理作用
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Concentric Gel System to Study the Biophysical Role of Matrix Microenvironment on 3D Cell Migration
Please note that all translations are automatically generated.
Click here for the English version.
同心凝胶系统来研究基质微环境的三维细胞迁移的生物物理作用
DOI:
10.3791/52735-v
•
11:43 min
•
April 03, 2015
•
Nicholas Agung Kurniawan
,
Parthiv Kant Chaudhuri
,
Chwee Teck Lim
3
1
Systems Biophysics Department
,
FOM Institute AMOLF
,
2
Mechanobiology Institute
,
National University of Singapore
,
3
Department of Biomedical Engineering
,
National University of Singapore
Chapters
00:05
Title
01:22
Harvesting Cells
02:32
Preparing the Collagen Solutions
03:52
Formation of Concentric Gel Cultures
06:54
Live-Cell Imaging of Concentric Gel Cultures
08:16
Tracking Cell Movement
09:38
Results: Cell Movement Analysis
11:03
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
机械性能和细胞外基质的组织强烈地影响细胞的三维迁移。
的体外
方法来研究在生物物理变量的环境中,在人口和单个细胞水平的时空细胞迁移行为,进行说明。
Tags
3D Cell Migration
Cell Invasion
Extracellular Matrix
Mechanosensing
Breast Cancer Cells
Collagen Gels
Live-cell Imaging
Biophysical Microenvironment
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