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Chapters
- 00:05Title
- 00:52Culture Inoculation
- 03:00Cell Maintenance: Checking Cell Density and Viability and Passaging Cells
- 05:11Transfection
- 07:50Expression and Harvest
- 08:36Results: High Yield Expression of Appropriately Glycosylated Protein with Transient Transfection
- 10:20Conclusion
Laboratory-scale production of eukaryotic proteins with appropriate post-translational modification represents a significant barrier. Here is a robust protocol with rapid establishment and turnaround for protein expression using a mammalian expression system. This system supports selective amino acid, selective labeling of proteins and small molecule modulators of glycan composition.
