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Developmental Biology

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Kvantitativ analyse af Protein Expression til Studieinformationen Lineage Specification i Mouse præimplantationsperioden Embryoner
 

Kvantitativ analyse af Protein Expression til Studieinformationen Lineage Specification i Mouse præimplantationsperioden Embryoner

Article doi: 10.3791/53654
February 22nd, 2016 Usage Statistics

Summary February 22nd, 2016

Please note that all translations are automatically generated.

Click here for the English version.

Denne protokol viser en metode til at udføre kvantitativ, encellede in situ analyse af proteinekspression at studere afstamning specifikation i mus præimplantations embryoer. De procedurer, der er nødvendige til indsamling af blastocyster, hel-mount immunofluorescent påvisning af proteiner, er billeddannelse af prøver på en konfokal mikroskop, og nuklear segmentering og billedanalyse beskrevet.

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