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の開発と特性評価<em>インビトロ</em>微小血管ネットワークおよび内皮の定量的測定の[Ca<sup> 2+</sup>]<sub>私</sub>と一酸化窒素の生産
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JoVE Journal Biology
Development and Characterization of In Vitro Microvessel Network and Quantitative Measurements of Endothelial [Ca2+]i and Nitric Oxide Production
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の開発と特性評価<em>インビトロ</em>微小血管ネットワークおよび内皮の定量的測定の[Ca<sup> 2+</sup>]<sub>私</sub>と一酸化窒素の生産

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8,452 Views

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09:39 min

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May 19, 2016

DOI:

10.3791/54014-v

DOI:
10.3791/54014-v

•

09:39 min
May 19, 2016

•

2 Views
, , ,
1Department of Cellular and Molecular Physiology,College of Medicine, Penn State University, 2Lane Department of Computer Science and Electrical Engineering,West Virginia University

Summary

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Primary human umbilical vein endothelial cells (HUVECs) were grown to confluence within a microfluidic network device. The endothelial cell junction and F-actin distributions were illustrated and the changes in intracellular calcium concentration and nitric oxide production in response to adenosine triphosphate (ATP) were quantified in real-time at individual cell levels.

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Cite this Article

Xu, S., Li, X., Liu, Y., He, P. Development and Characterization of In Vitro Microvessel Network and Quantitative Measurements of Endothelial [Ca2+]i and Nitric Oxide Production. J. Vis. Exp. (111), e54014, doi:10.3791/54014 (2016).

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