Desarrollo y caracterización de<em> In Vitro</em> Red de microvasos y mediciones cuantitativas de endotelial [Ca<sup> 2+</sup>]<sub> i</sub> Y la producción de óxido nítrico
Primary human umbilical vein endothelial cells (HUVECs) were grown to confluence within a microfluidic network device. The endothelial cell junction and F-actin distributions were illustrated and the changes in intracellular calcium concentration and nitric oxide production in response to adenosine triphosphate (ATP) were quantified in real-time at individual cell levels.