Desenvolvimento e Caracterização de<em> In Vitro</em> Rede de microvasos e medições quantitativas de endotelial [ca<sup> 2+</sup>]<sub> i</sub> E óxido nítrico Produção
Primary human umbilical vein endothelial cells (HUVECs) were grown to confluence within a microfluidic network device. The endothelial cell junction and F-actin distributions were illustrated and the changes in intracellular calcium concentration and nitric oxide production in response to adenosine triphosphate (ATP) were quantified in real-time at individual cell levels.