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Immunology and Infection
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Generazione di Immaturo, matura e tollerogenico dendritiche cellule con differenti fenotipi metabolica
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Generation of Immature, Mature and Tolerogenic Dendritic Cells with Differing Metabolic Phenotypes
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Generazione di Immaturo, matura e tollerogenico dendritiche cellule con differenti fenotipi metabolica

DOI:
10.3791/54128-v

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06:09 min

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June 22, 2016

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, , ,
1Translational Immunology, Institute of Molecular and Cell Biology,Agency for Science, Technology and Research, 2Singapore Immunology Network, 3Institute of Biomedical Studies,Baylor University

Chapters

  • 00:05Title
  • 00:37Monocyte Enrichment by Magnetic Separation and Magnetic Labeling
  • 01:35Magnetic Separation
  • 02:44Generating Different moDCs
  • 04:17Results: Enhanced Characterization and Analysis of moDCs
  • 05:39Conclusion

Summary

Automatic Translation

Automatic Translation

Immature dendritic cells can be selectively differentiated into tolerogenic or mature dendritic cells to regulate the balance between immunity and tolerance. This work presents a means to generate from immature monocyte derived dendritic cells (moDCs), in vitro tolerogenic and mature moDCs that differ in metabolic phenotypes.

Tags

Keywords: Dendritic CellsMonocyte EnrichmentCD14+ CellsImmature Dendritic CellsMature Dendritic CellsTolerogenic Dendritic CellsMetabolic PhenotypesCell CultureIn VitroImmunologyVaccine Development

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