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Påvisning af RNA-bindende proteiner ved

Article DOI: 10.3791/54207-v • 10:34 min • July 22nd, 2016
July 22nd, 2016 •
*1, *1, 3, 1,2
1Cardiovascular and Metabolic Disorders Program, Duke-NUS Medical School, 2Institute of Molecular and Cell Biology, Singapore, 3Division of Bioengineering, School of Chemical & Biomedical Engineering, Nanyang Technological University
* These authors contributed equally

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En RNA pull-down protokol er optimeret her til påvisning af interaktioner mellem RNA-bindende proteiner (RBP'er) og ikke-kodende samt kodende RNA'er. En RNA-fragment fra androgen receptor (AR) blev anvendt som et eksempel for at vise hvordan man kan hente sin RBP fra lystate af primære brune fedtceller.

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Molekylærbiologi RNA pull-down RNA-bindende protein fedtcellerne ikke-kodende kodning
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