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1Singapore Immunology Network (SIgN),Agency for Science, Technology and Research (A*STAR), Biopolis, 2Department of Microbiology and Immunology, Yong Loo Lin School of Medicine,National University of Singapore, 3Lee Kong Chian School of Medicine,Nanyang Technological University, 4Centenary Institute for Cancer Medicine and Cell Biology, 5Discipline of Dermatology,University of Sydney, 6Department of Dermatology,Royal Prince Alfred Hospital, 7LSI Immunology Programme,National University of Singapore, 8School of Biological Sciences,Nanyang Technological University
Chapters
- 00:05Title
- 00:53Mouse Anesthesia and Depilation
- 01:35Induction of Ischemia and Reperfusion Injury
- 02:31Placement of the Ear on the Imaging Platform
- 04:33Results: Neutrophil Recruitment in Ischemia-reperfusion Injury
- 05:20Conclusion
Denne protokollen beskriver induksjon av en ischemi-reperfusjon (IR) modell på museøre huden ved hjelp av magnet fastspenning. Ved hjelp av en spesialbygd intra bildebehandling modell, studerer vi in vivo inflammatoriske responser etter reperfusjon. Begrunnelsen bak utviklingen av denne teknikken er å utvide forståelsen av hvordan leukocytter svare på huden IR skade.
