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En Fluorescens-baserede lymfocyt Assay Egnet til high-throughput screening af små molekyler
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules
DOI:

08:43 min

March 10, 2017

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Chapters

  • 00:05Title
  • 00:40Generation of Splenocyte Cell Suspension from Nur77GFP Mouse Spleens
  • 02:07T Cell Isolation from the Splenocyte Cell Suspension
  • 03:26T-cell Activation and the Induction of GFP Expression
  • 04:42High Throughput Screening of Small Molecule
  • 06:05Results: T and B Cell Receptors Activation Flowcytometry Results and a Representative T Cell HTS of Smal Molecules
  • 07:56Conclusion

Summary

Automatic Translation

Vi præsenterer i den aktuelle undersøgelse en hidtil ukendt fluorescens-baseret assay under anvendelse af lymfocytter afledt fra en transgen mus. Dette assay er egnet til high-throughput screening (HTS) af små molekyler forsynet med kapacitet på enten inhibere eller fremme lymfocytaktivering.

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