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En Fluorescens-baserede lymfocyt Assay Egnet til high-throughput screening af små molekyler
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Chapters
- 00:05Title
- 00:40Generation of Splenocyte Cell Suspension from Nur77GFP Mouse Spleens
- 02:07T Cell Isolation from the Splenocyte Cell Suspension
- 03:26T-cell Activation and the Induction of GFP Expression
- 04:42High Throughput Screening of Small Molecule
- 06:05Results: T and B Cell Receptors Activation Flowcytometry Results and a Representative T Cell HTS of Smal Molecules
- 07:56Conclusion
Vi præsenterer i den aktuelle undersøgelse en hidtil ukendt fluorescens-baseret assay under anvendelse af lymfocytter afledt fra en transgen mus. Dette assay er egnet til high-throughput screening (HTS) af små molekyler forsynet med kapacitet på enten inhibere eller fremme lymfocytaktivering.
