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Küçük moleküllerin yüksek verimli tarama için uygun bir floresan bazlı lenfositi analizi
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules
DOI:

08:43 min

March 10, 2017

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Chapters

  • 00:05Title
  • 00:40Generation of Splenocyte Cell Suspension from Nur77GFP Mouse Spleens
  • 02:07T Cell Isolation from the Splenocyte Cell Suspension
  • 03:26T-cell Activation and the Induction of GFP Expression
  • 04:42High Throughput Screening of Small Molecule
  • 06:05Results: T and B Cell Receptors Activation Flowcytometry Results and a Representative T Cell HTS of Smal Molecules
  • 07:56Conclusion

Summary

Automatic Translation

Biz bu çalışmada bir transgenik fareden türetilen lenfositlerin kullanılarak yeni floresans bazlı analiz sunuyoruz. Bu deney, inhibe veya lenfosit aktivasyonunu teşvik ya kapasitesine sahip küçük moleküller, yüksek verimli tarama (HTS) için uygundur.

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