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基于荧光的淋巴细胞检测适用于小分子的高通量筛选
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules
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基于荧光的淋巴细胞检测适用于小分子的高通量筛选

DOI:
10.3791/55199-v

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08:43 min

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March 10, 2017

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, , , ,
1Department of Microbiology, Infectiology and Immunology,Université de Montréal, 2Department of Pharmacology and Physiology,Université de Montréal, 3Molecular Biology Program,Université de Montréal

Chapters

  • 00:05Title
  • 00:40Generation of Splenocyte Cell Suspension from Nur77GFP Mouse Spleens
  • 02:07T Cell Isolation from the Splenocyte Cell Suspension
  • 03:26T-cell Activation and the Induction of GFP Expression
  • 04:42High Throughput Screening of Small Molecule
  • 06:05Results: T and B Cell Receptors Activation Flowcytometry Results and a Representative T Cell HTS of Smal Molecules
  • 07:56Conclusion

Summary

Automatic Translation

Automatic Translation

我们提出在目前的研究使用从转基因小鼠衍生的淋巴细胞的新颖的基于荧光的测定法。该测定是适于赋予的任抑制或促进淋巴细胞的活化的能力的小分子的高通量筛选（HTS）。

Tags

Fluorescence-based Lymphocyte AssayHigh-throughput ScreeningSmall Molecule ImmunomodulatorsLymphocyte Activation And InhibitionPhenotypic Function AssayDrug DiscoveryNurr77 GFP MouseSplenocyte IsolationT Cell IsolationMagnetic Bead Separation

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