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Culture of Adult transgene zebrafisk Retinal Eksplantater til Live-celle Imaging af multifoton Microscopy
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Culture of Adult Transgenic Zebrafish Retinal Explants for Live-cell Imaging by Multiphoton Microscopy
DOI:

11:55 min

February 24, 2017

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Chapters

  • 00:05Title
  • 01:11Isolation and Culturing of Retinal Explants
  • 03:30Multiphoton Microscopy
  • 07:52Analysis of Velocity
  • 09:34Results: Analysis of Apical and Basal Migration Velocities
  • 10:55Conclusion

Summary

Automatic Translation

Zebrafisk retinal regenerering er for det meste blevet undersøgt ved hjælp af faste nethinder. Imidlertid dynamiske processer såsom interkinetic nuklear migration forekomme under den regenerative respons og kræver live-cell imaging at undersøge de underliggende mekanismer. Her beskriver vi kultur og billedbehandling betingelser for at overvåge Interkinetic Nuclear Migration (INM) i realtid ved hjælp multifoton mikroskopi.

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