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JoVE Journal
Developmental Biology

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成年转基因斑马鱼视网膜外植体培养由多光子显微镜活细胞成像
 
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成年转基因斑马鱼视网膜外植体培养由多光子显微镜活细胞成像

Article DOI: 10.3791/55335
February 24th, 2017

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Summary February 24th, 2017

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斑马鱼视网膜再生大多被使用固定的视网膜研究。然而,再生反应过程中发生的动态过程,如interkinetic核迁移,并需要活细胞成像研究的基本机制。这里,我们描述培养和成像条件下,使用多光子显微术来监控Interkinetic核迁移(INM)的实时性。

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