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Protokoller for Visualisering Steroidogenic organer og deres Interaktiv organer med Farging i bananflue<em> Drosophila melanogaster</em
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Protocols for Visualizing Steroidogenic Organs and Their Interactive Organs with Immunostaining in the Fruit Fly Drosophila melanogaster
DOI:

12:10 min

April 14, 2017

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Chapters

  • 00:05Title
  • 00:57Dissecting the Larval Ring Gland-brain Complex
  • 01:53Filleting Larvae to Maintain Relative Tissue Positions
  • 03:35Fine Dissection of the Brain-ring Gland Complex and Mounting
  • 05:34Dissection of Adult Ovary
  • 06:36Dissection of Adult Reproductive Organs Along with Other Interactive Tissues
  • 08:15Mounting of Adult Structures and Imaging
  • 09:33Results: Preparation images
  • 11:13Conclusion

Summary

Automatic Translation

Vi beskriver en protokoll for disseksjon, fiksering og immunofarging av steroidogenic organer i Drosophila-larver og voksne kvinner for å studere steroid hormon biosyntese og dens reguleringsmekanisme. I tillegg til steroidogenic organer, visual vi innervasjon av steroidogenic organer samt steroidogenic målceller slik som kimlinje-stamceller.

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