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神经肽 Y-pHluorin 的共焦成像
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神经肽 Y-pHluorin 的共焦成像: 一种完整的小鼠和人胰岛胰岛素颗粒胞的可视化技术

Article DOI: 10.3791/56089-v 09:41 min September 13th, 2017
September 13th, 2017



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Click here for the English version.

我们描述了一个在完整的胰岛使用 pHluorin, pH 敏感的绿色荧光蛋白可视化的胞的协议。孤立的胰岛感染腺病毒编码 pHluorin 耦合到囊泡货物神经肽 Y。这使得检测胰岛素颗粒融合事件的共聚焦显微镜。


细胞生物学 问题 127 胰岛素颗粒 神经肽 Y pHluorin 搏动分泌 胰岛 腺病毒
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