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September 13, 2017
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Biz pHluorin, pH-duyarlı yeşil flüoresan protein kullanarak bozulmamış adacıkları insülin ekzositozu görselleştirme için bir iletişim kuralı tanımlamak. İzole adacıkları adenovirus pHluorin vezikül kargo peptit Y birleştiğinde kodlama ile bulaşmış. Bu confocal mikroskobu tarafından insülin granül füzyon olayları algılama için sağlar.
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Cite this Article
Makhmutova, M., Liang, T., Gaisano, H., Caicedo, A., Almaça, J. Confocal Imaging of Neuropeptide Y-pHluorin: A Technique to Visualize Insulin Granule Exocytosis in Intact Murine and Human Islets. J. Vis. Exp. (127), e56089, doi:10.3791/56089 (2017).
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