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JoVE Journal
Biology

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Konfokal Imaging af neuropeptid Y-pHluorin
 
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Konfokal Imaging af neuropeptid Y-pHluorin: en teknik til at visualisere Insulin granulet exocytose i intakt Murine og menneskelige Holme

Article DOI: 10.3791/56089-v 09:41 min September 13th, 2017
September 13th, 2017

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Summary

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Vi beskriver en protokol for visualisering af insulin exocytose i intakt Holme ved hjælp af pHluorin, en pH-følsom grøn fluorescerende proteiner. Isolerede øer er inficeret med adenovirus kodning pHluorin koblet til vesikel cargo neuropeptid Y. Dette giver mulighed for påvisning af insulin granulet fusion begivenheder af Konfokal mikroskopi.

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Cellebiologi sag 127 Insulin granula exocytose neuropeptid Y pHluorin pulsatile sekretionen pancreas Holme adenovirus
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