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Developmental Biology
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在体内
荧光亲脂膜染料对大鼠膝骨关节炎模型中人脂肪间充质干细胞的追踪
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JoVE Journal
Developmental Biology
In Vivo
Tracking of Human Adipose-derived Mesenchymal Stem Cells in a Rat Knee Osteoarthritis Model with Fluorescent Lipophilic Membrane Dye
Please note that all translations are automatically generated.
Click here for the English version.
在体内
荧光亲脂膜染料对大鼠膝骨关节炎模型中人脂肪间充质干细胞的追踪
DOI:
10.3791/56273-v
•
07:31 min
•
October 08, 2017
•
Meng Li*
1,2
,
Ming Hao*
1,2
,
Dong Jiang
,
Yanxi Chen
,
Wen Wang
2
1
Cellular Biomedicine Group, China
,
2
Cellular Biomedicine Group, California
,
3
Department of Orthopedics
,
Shanghai TCM-Integrated Hospital
,
4
Department of Orthopedics, Shanghai East Hospital
,
Tongji University
Chapters
00:05
Title
00:58
Surgical Rat Knee Osteoarthritis (KOA) Induction
02:36
Human Mesenchymal Stem Cell Fluorescent Dye Labelling and
In Vivo
Fluorescent Imaging
05:16
Results: Representative Rat KOA Induction and haMSC Imaging Analyses
06:49
Conclusion
Summary
Automatic Translation
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Automatic Translation
该协议描述了一种有效的方法来监测人脂肪来源的骨髓间充质干细胞 (haMSCs) 的细胞持久性和分布的远红色荧光标记的大鼠膝骨关节炎 (KOA) 模型通过关节内 (IA) 注射。
Tags
In Vivo Tracking
Human Adipose-derived Mesenchymal Stem Cells
Rat Knee Osteoarthritis Model
Fluorescent Lipophilic Membrane Dye
DiD
Stem Cell Persistence
Biodistribution
Regenerative Medicine
Animal Model
Cell Labeling
In Vivo Imaging
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