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透射电镜下体的样品制备与成像
JoVE Journal
Biology
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JoVE Journal
Biology
Sample Preparation and Imaging of Exosomes by Transmission Electron Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
透射电镜下体的样品制备与成像
DOI:
10.3791/56482-v
•
11:15 min
•
January 04, 2018
•
Min Kyo Jung
,
Ji Young Mun
1
Department of Convergence Medicine, University of Ulsan College of Medicine and Asan Institute for Life Sciences
,
Asan Medical Center
,
2
Synaptic Circuit Plasticity Laboratory, Department of Structure and Function of Neural Network
,
Korea Brain Research Institute
Chapters
00:05
Title
01:14
Exosome Block Preparation
03:38
Sectioning, Staining, and Imaging of the Exosome
04:27
Immuno-staining of Sections and Imaging
07:24
Whole Mount for Immunostaining
09:10
Results: Immunostained Exosome Sections Provide Insight to Their Protein Makeup
09:59
Conclusion
Summary
Automatic Translation
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Automatic Translation
本协议描述了传输电子显微镜所需的各种技术, 包括阴性染色, 超薄切片的详细结构, 和免疫金标签, 以确定特定的蛋白质在体的位置。
Tags
Exosomes
Transmission Electron Microscopy
Sample Preparation
Imaging
Extracellular Vesicles
Protein Localization
Ultracentrifugation
Fixation
Dehydration
Embedding
Low Viscosity Embedding
Disease Diagnosis
Microcellular Incubation
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