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Probenvorbereitung und Bildgebung von Exosomen von Transmissions-Elektronenmikroskopie
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Biology
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JoVE Journal Biology
Sample Preparation and Imaging of Exosomes by Transmission Electron Microscopy
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Probenvorbereitung und Bildgebung von Exosomen von Transmissions-Elektronenmikroskopie

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37,426 Views

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11:15 min

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January 04, 2018

DOI:

10.3791/56482-v

DOI:
10.3791/56482-v

•

11:15 min
January 04, 2018

•

39 Views
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1Department of Convergence Medicine, University of Ulsan College of Medicine and Asan Institute for Life Sciences,Asan Medical Center, 2Synaptic Circuit Plasticity Laboratory, Department of Structure and Function of Neural Network,Korea Brain Research Institute

Summary

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Dieses Protokoll beschreibt die verschiedenen Techniken für Transmissions-Elektronenmikroskopie, einschließlich negative Färbung, ultradünnen schneiden für detaillierte Struktur und Immuno-Gold um die Positionen der spezifischen Proteine in Exosomen bestimmen Kennzeichnung notwendig.

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Cite this Article

Jung, M. K., Mun, J. Y. Sample Preparation and Imaging of Exosomes by Transmission Electron Microscopy. J. Vis. Exp. (131), e56482, doi:10.3791/56482 (2018).

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