Journal
/
/
Eksempel forberedelse og bildebehandling for Exosomes av overføring elektronmikroskop
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Sample Preparation and Imaging of Exosomes by Transmission Electron Microscopy
DOI:

11:15 min

January 04, 2018

,

Chapters

  • 00:05Title
  • 01:14Exosome Block Preparation
  • 03:38Sectioning, Staining, and Imaging of the Exosome
  • 04:27Immuno-staining of Sections and Imaging
  • 07:24Whole Mount for Immunostaining
  • 09:10Results: Immunostained Exosome Sections Provide Insight to Their Protein Makeup
  • 09:59Conclusion

Summary

Automatic Translation

Denne protokollen beskriver ulike teknikker nødvendig for overføring elektronmikroskop inkludert negative flekker, supertynn snitting for detaljert struktur og immun-gull merking for å bestemme plasseringen av spesifikke proteiner i exosomes.

Related Videos

Read Article