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Journal
/
Developmental Biology
/
细胞聚集法评价
果蝇
切口与
反式
配体的结合及其对
顺
式配体的抑制作用
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Cell Aggregation Assays to Evaluate the Binding of the
Drosophila
Notch with
Trans
-Ligands and its Inhibition by
Cis
-Ligands
Please note that all translations are automatically generated.
Click here for the English version.
细胞聚集法评价
果蝇
切口与
反式
配体的结合及其对
顺
式配体的抑制作用
DOI:
10.3791/56919-v
•
05:48 min
•
January 02, 2018
•
Ashutosh Pandey
,
Hamed Jafar-Nejad
2
1
Department of Molecular and Human Genetics
,
Baylor College of Medicine
,
2
Program in Developmental Biology
,
Baylor College of Medicine
Chapters
00:05
Title
00:38
Assessment of Binding Between Notch Receptor and
Trans
-ligands
01:47
Aggregation Between Signal-sending and Signal-receiving Cells
03:11
Evaluation of Inhibition of Binding Between Notch and
Trans
-ligands by
Cis
-ligands
04:15
Results: Faithful Analysis of Notch Signaling
05:30
Conclusion
Summary
Automatic Translation
English (Original)
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français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
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Automatic Translation
复杂的
在体内
系统, 使得很难区分的激活和抑制的缺口受体的
跨
-和
cis
配体, 分别。在这里, 我们提出了一个基于
体外
细胞聚合的方法, 用于定性和半定量评价的结合的
果蝇
缺口, 以
跨
配基与
顺
式配基。
Tags
Cell Aggregation Assay
Notch Receptor
Trans-ligands
Cis-ligands
Drosophila
S2 Cells
Ligand Binding
Cell Signaling
Aggregation Quantification
Microscopy
Hemacytometer
Article
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