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Journal
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Immunology and Infection
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人雌性生殖道树突状细胞表型鉴定的分离及功能研究
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Isolation of Dendritic Cells from the Human Female Reproductive Tract for Phenotypical and Functional Studies
Please note that all translations are automatically generated.
Click here for the English version.
人雌性生殖道树突状细胞表型鉴定的分离及功能研究
DOI:
10.3791/57100-v
•
12:32 min
•
March 13, 2018
•
Marta Rodriguez-Garcia
,
Jared M. Fortier
,
Fiona D. Barr
,
Charles R. Wira
1
Department of Microbiology and Immunology
,
Geisel School of Medicine at Dartmouth
Chapters
00:04
Title
01:07
Enzymatic Digestion of Tissues
02:34
Physical Separation of Single Cells
04:57
Dead Cell Removal
06:00
DC Purification by Positive Magnetic Bead Selection
08:02
Allogeneic Stimulation Assay to Assess DC Cell Function
10:19
Results: Successful Isolation of DCs from the Human Female Reproductive Tract
11:55
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文介绍了一种从人类女性生殖道的不同解剖间隔分离纯化树突状细胞的方法, 以评价其表型鉴定和功能特征。该方法可用于隔离其他粘膜组织中的其他免疫细胞或树突状细胞。
Tags
Dendritic Cells
Female Reproductive Tract
Phenotypical
Functional
Immune Cells
Epithelial Sheets
Enzymatic Digestion
Cell Isolation
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