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分离块茎原生质体流式细胞仪测量 Endoreduplication 的研究
JoVE Journal
Biology
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JoVE Journal
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Measuring Endoreduplication by Flow Cytometry of Isolated Tuber Protoplasts
Please note that all translations are automatically generated.
Click here for the English version.
分离块茎原生质体流式细胞仪测量 Endoreduplication 的研究
DOI:
10.3791/57134-v
•
08:54 min
•
March 09, 2018
•
F. Parker E. Laimbeer
,
Melissa Makris
,
Richard E. Veilleux
1
Department of Horticulture
,
Virginia Tech
,
2
Department of Biomedical Sciences and Pathobiology, Center for Molecular Medicine and Infectious Diseases, Virginia-Maryland Regional College of Veterinary Medicine
,
Virginia Tech
Chapters
00:05
Title
00:36
Day 1: Plasmolyze Tuber Tissue
01:40
Days 2 and 3: Generate Potato Protoplasts and Harvest and Wash Protoplasts
03:42
Day 3: Prepare Samples for Flow Cytometry
05:07
Day 3: Flow Cytometry of Potato Tuber Nuclei
06:31
Results: Endoreduplication of Isolated Tuber Protoplasts
08:07
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文描述的协议是一种测量马铃薯块茎内 endoreduplication 的方法 (
马铃薯
)。它包括质壁分离和原生质体提取步骤, 以减少下游流细胞分析中的噪声和碎片。
Tags
Endoreduplication
Flow Cytometry
Potato Tubers
Protoplasts
Cell Morphology
Developmental Stage
Plasmolysis
Enzyme Solution
Cellulase
Macerozyme
Hemicellulase
MES Buffer
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