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Måling af endoreduplikation ved flowcytometri af isolerede knold Protoplasts
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Biology
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JoVE Journal Biology
Measuring Endoreduplication by Flow Cytometry of Isolated Tuber Protoplasts

Måling af endoreduplikation ved flowcytometri af isolerede knold Protoplasts

DOI:
10.3791/57134-v

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08:54 min

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March 09, 2018

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, ,
1Department of Horticulture,Virginia Tech, 2Department of Biomedical Sciences and Pathobiology, Center for Molecular Medicine and Infectious Diseases, Virginia-Maryland Regional College of Veterinary Medicine,Virginia Tech

Chapters

  • 00:05Title
  • 00:36Day 1: Plasmolyze Tuber Tissue
  • 01:40Days 2 and 3: Generate Potato Protoplasts and Harvest and Wash Protoplasts
  • 03:42Day 3: Prepare Samples for Flow Cytometry
  • 05:07Day 3: Flow Cytometry of Potato Tuber Nuclei
  • 06:31Results: Endoreduplication of Isolated Tuber Protoplasts
  • 08:07Conclusion

Summary

Automatic Translation

Automatic Translation

Protokollen beskrevet heri er en metode til måling af endoreduplikation inden for knolde af kartoffel (Solanum tuberosum). Det omfatter plasmolysis og protoplast udvinding skridt for at mindske støj og vragrester i downstream flow cytometric analyse.

Tags

EndoreduplicationFlow CytometryPotato TubersProtoplastsCell MorphologyDevelopmental StagePlasmolysisEnzyme SolutionCellulaseMacerozymeHemicellulaseMES Buffer

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