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Måling af endoreduplikation ved flowcytometri af isolerede knold Protoplasts
JoVE Journal
Biology
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JoVE Journal Biology
Measuring Endoreduplication by Flow Cytometry of Isolated Tuber Protoplasts
DOI:

08:54 min

March 09, 2018

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Chapters

  • 00:05Title
  • 00:36Day 1: Plasmolyze Tuber Tissue
  • 01:40Days 2 and 3: Generate Potato Protoplasts and Harvest and Wash Protoplasts
  • 03:42Day 3: Prepare Samples for Flow Cytometry
  • 05:07Day 3: Flow Cytometry of Potato Tuber Nuclei
  • 06:31Results: Endoreduplication of Isolated Tuber Protoplasts
  • 08:07Conclusion

Summary

Automatic Translation

Protokollen beskrevet heri er en metode til måling af endoreduplikation inden for knolde af kartoffel (Solanum tuberosum). Det omfatter plasmolysis og protoplast udvinding skridt for at mindske støj og vragrester i downstream flow cytometric analyse.

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