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Immunology and Infection
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腹膜源性肥大细胞的分离及其功能特征的 Ca
2 +
成像和脱粒测定
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca
2+
-imaging and Degranulation Assays
Please note that all translations are automatically generated.
Click here for the English version.
腹膜源性肥大细胞的分离及其功能特征的 Ca
2 +
成像和脱粒测定
DOI:
10.3791/57222-v
•
11:31 min
•
July 04, 2018
•
Volodymyr Tsvilovskyy
,
Alejandra Solis-Lopez
,
Kathrin Öhlenschläger
,
Marc Freichel
1
Institute of Pharmacology
,
Ruprecht-Karls Heidelberg University
Chapters
00:04
Title
00:38
PMC Isolation and Cultivation by Intraperitoneal Lavage
03:14
Fluorometric Intracellular Free Calcium Concentration Measurement in PMCs
06:38
Beta-hexosaminidase Release Measurement in PMCs
08:46
Results: Beta-hexosaminidase Release Colorimetric Multi-well Measurements Performed with PMCs Isolated from Wild Type Mice
10:14
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们提出了一个隔离和培养小鼠腹膜肥大细胞的协议。我们还描述了两个协议的功能表征: 一个荧光成像的细胞内游离 Ca
2 +
浓度和脱粒检测的基础上的色度量化的释放β-hexosaminidase。
Tags
Peritoneum
Mast Cells
Calcium Imaging
Degranulation
Intracellular Signaling
Excitable Cells
Ion Channels
Immune Cell Activation
Mediator Release
Fura-2
RPMI Medium
Peritoneal Cavity
Cell Suspension
IL-3
SCF
IgE Receptors
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