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Calorespirometry: 一种功能强大、无创的研究细胞能量代谢的方法
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Calorespirometry: A Powerful, Noninvasive Approach to Investigate Cellular Energy Metabolism
DOI:

09:08 min

May 31, 2018

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Chapters

  • 00:04Title
  • 00:51Respirometer and Colorimeter Preparation
  • 02:25Human Hepatocellular Carcinoma (HepG2) Cell Preparation
  • 04:26Calorimetry and Respirometry
  • 06:36Results: Representative O2 Concentration and Flux Analyses
  • 08:23Conclusion

Summary

Automatic Translation

该协议描述 calorespirometry, 直接和同时测量散热和呼吸, 这提供了一种无创的方法来评估能量代谢。该技术用于通过监测总的细胞能量流来评估有氧和厌氧途径对能源利用的贡献。

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