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Bioengineering
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Calorespirometry: 一种功能强大、无创的研究细胞能量代谢的方法
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Calorespirometry: A Powerful, Noninvasive Approach to Investigate Cellular Energy Metabolism
Please note that all translations are automatically generated.
Click here for the English version.
Calorespirometry: 一种功能强大、无创的研究细胞能量代谢的方法
DOI:
10.3791/57724-v
•
09:08 min
•
May 31, 2018
•
Robert A. Skolik
,
Mary E. Konkle
,
Michael A. Menze
1
Department of Biological Sciences
,
University of Louisville
,
2
Department of Chemistry
,
Ball State University
Chapters
00:04
Title
00:51
Respirometer and Colorimeter Preparation
02:25
Human Hepatocellular Carcinoma (HepG2) Cell Preparation
04:26
Calorimetry and Respirometry
06:36
Results: Representative O
2
Concentration and Flux Analyses
08:23
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述 calorespirometry, 直接和同时测量散热和呼吸, 这提供了一种无创的方法来评估能量代谢。该技术用于通过监测总的细胞能量流来评估有氧和厌氧途径对能源利用的贡献。
Tags
Calorespirometry
Cellular Energy Metabolism
Mitochondrial Toxicity
Pharmaceutical Development
Cryobiology
Respirometer
Calorimetry
Oxygen Consumption
Heat Production
HepG2 Cells
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