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1Lee Kong Chian School of Medicine,Nanyang Technological University, 2Singapore Eye Research Institute (SERI),Singapore General Hospital, 3The Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska Institutet,Karolinska University Hospital, 4Cancer and Stem Cell Biology Program,Duke-NUS Medical School, 5Department of Cell and Molecular Biology,Karolinska Institutet
Chapters
- 00:04Title
- 00:33Induction of Hypoxia in HEK293A Cells
- 01:28Harvesting Cells Cultured Under Hypoxic Conditions
- 03:30Preparing Nuclear Extracts
- 05:52Immunoprecipitation of Proteins
- 07:55Results: HIF-1α Interacts with ARNT at Hypoxia
- 08:56Conclusion
Nous décrivons ici un protocole co-immunoprécipitation pour étudier les interactions protéine-protéine entre les protéines nucléaires endogènes dans des conditions hypoxiques. Cette méthode convient pour la démonstration des interactions entre les facteurs de transcription et les corégulateurs transcriptionnels à l’hypoxie.
