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Biology
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缺氧条件下培养细胞内源性核蛋白的共免疫沉淀测定
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Biology
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JoVE Journal
Biology
Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions
Please note that all translations are automatically generated.
Click here for the English version.
缺氧条件下培养细胞内源性核蛋白的共免疫沉淀测定
DOI:
10.3791/57836-v
•
09:17 min
•
August 02, 2018
•
Xiaofeng Zheng
2
,
Calvin Qing Wei Ho
,
Xiaowei Zheng
,
Kian Leong Lee
,
Katarina Gradin
,
Teresa S. Pereira
,
Per-Olof Berggren
2,3
,
Yusuf Ali
2
1
Lee Kong Chian School of Medicine
,
Nanyang Technological University
,
2
Singapore Eye Research Institute (SERI)
,
Singapore General Hospital
,
3
The Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska Institutet
,
Karolinska University Hospital
,
4
Cancer and Stem Cell Biology Program
,
Duke-NUS Medical School
,
5
Department of Cell and Molecular Biology
,
Karolinska Institutet
Chapters
00:04
Title
00:33
Induction of Hypoxia in HEK293A Cells
01:28
Harvesting Cells Cultured Under Hypoxic Conditions
03:30
Preparing Nuclear Extracts
05:52
Immunoprecipitation of Proteins
07:55
Results: HIF-1α Interacts with ARNT at Hypoxia
08:56
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们描述了一个共同免疫沉淀协议, 研究在缺氧条件下内源核蛋白的蛋白质相互作用。该方法适用于低氧转录因子与转录联合调节剂相互作用的证明。
Tags
Co-immunoprecipitation
Endogenous Nuclear Proteins
Hypoxic Conditions
Transcription Factors
Transcriptional Coregulators
Human Embryonic Kidney 293A Cells
DPBS
Hypoxia Subchamber
Normoxia
Cell Culture
Cell Harvesting
Nuclear Fractions
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