A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:04Title
- 00:47Confocal Laser Scanning Microscopy
- 01:49Required Software Installation
- 02:45Image Analysis in Fiji
- 09:27Results: Quantification of Plasma Membrane Partitioning for Examined Samples
- 10:33Conclusion
Here, we present a protocol to perform a quantitative analysis of the level of plasma-membrane association for fluorescently-tagged peripherally-associated protein. The method is based on the computational decomposition of membrane and cytoplasmic component of signal observed in cells labeled with plasma membrane fluorescent marker.
