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June 15, 2018
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Nous présentons ici un protocole afin d’effectuer une analyse quantitative du niveau de l’association de la membrane plasmique fluorescent-étiquetées de protéine associée à la périphérie. La méthode est basée sur la décomposition computationnelle de membrane et composants cytoplasmiques du signal observé dans les cellules marquées avec la membrane plasmique marqueur fluorescent.
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Cite this Article
Vosolsobě, S., Schwarzerová, K., Petrášek, J. Determination of Plasma Membrane Partitioning for Peripherally-associated Proteins. J. Vis. Exp. (136), e57837, doi:10.3791/57837 (2018).
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