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Fastsettelse av Plasma membranen partisjonering for perifert knyttet proteiner
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Fastsettelse av Plasma membranen partisjonering for perifert knyttet proteiner

Article DOI: 10.3791/57837-v • 11:11 min • June 15th, 2018
June 15th, 2018 •
1, 1, 1
1Department of Experimental Plant Biology, Faculty of Science, Charles University

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Her presenterer vi en protokoll for å utføre en kvantitativ analyse av nivået av plasma-membran foreningen for fluorescently-merket perifert knyttet protein. Metoden er basert på beregningsorientert nedbryting av membran og cytoplasmatiske komponent signal i celler merket med plasma membranen fluorescerende markør.

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Biologi problemet 136 perifert knyttet plasma membran proteiner membran affinitet membran partisjonering AC confocal mikroskopi ImageJ R-project FM 4-64 deconvolution
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