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June 15, 2018
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Her presenterer vi en protokoll for å utføre en kvantitativ analyse av nivået av plasma-membran foreningen for fluorescently-merket perifert knyttet protein. Metoden er basert på beregningsorientert nedbryting av membran og cytoplasmatiske komponent signal i celler merket med plasma membranen fluorescerende markør.
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Cite this Article
Vosolsobě, S., Schwarzerová, K., Petrášek, J. Determination of Plasma Membrane Partitioning for Peripherally-associated Proteins. J. Vis. Exp. (136), e57837, doi:10.3791/57837 (2018).
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